B. cereus was grown on urea agar for 6 days at 37 degrees C to induce spore formation.
The endospore stain was done using malachite green as the primary stain and safranin as counter the stain. Malachite green was applied to the smear and set over a steaming water bath for 10 min (stain was not allowed to dry out), rinsed gently with DI water, then safranin added for several minutes (not over water bath).
A). Endospore’s stain blue, still in the vegetative cells which stains red (parent cell).
B). The vegetative cell, stains red.
C). Spores that have been released from the vegetative cell