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Lipid Agar

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Lipid Plate/Tributyrin Agar used to test for an organisms ability to produce the exoenzyme lipase which breaks down the lipids in the agar creating a clear zone around the organism. (A) Serratia marcesens, lipid hydrolysis, indicated by a zone of clearing around the growing colony, as well as the red colony pigment prodigiosin. (B) E. coli, no lipid hydrolysis (C) Pseudomonas aeruginosa, lipid hydrolysis, indicated by a zone of clearing around the growing colony, as well as the slight green diffusable pigment pyocyanin Note: pigment production is a characteristic of the organism and not influenced by the lipid media.
 
 

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