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Click-Chemistry Labeling of Oligonucleotides and DNA - Lumiprobe - Protocol


Click chemistry is a versatile reaction that can be used for the synthesis of a variety of conjugates. Virtually any biomolecules can be involved, and labeling with small molecules, such as fluorescent dyes, biotin, and other groups can be readily achieved.

Click chemistry reaction takes place between two components: azide and alkyne (terminal acetylene). Both azido and alkyne groups are nearly never encountered in natural biomolecules. Hence, the reaction is highly bioorthogonal and specific. If there is a need to label an oligonucleotide, alkyne-modified oligonucleotides can be ordered at many of the custom oligo-synthesizing facilites and companies.

Lumiprobe recommends using the following general protocol for Click chemistry labeling of alkyne-modified oligonucleotides with azides produced by Lumiprobe Corp. The auxiliary reagents can be ordered at Lumiprobe Corp..

Continue reading protocol...Calculate the volumes of reagents required for Click chemistry labeling using the table below. Prepare the required stock solutions ........

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Lumiprobe.com offers cyanine dyes and explains the difference. Free Tech support is available to help you determine what to use for your research.

Summary of what each reagent does

Ideas- Lumiprobe citation list

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