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Cyanine dyes - Non-sulfonated and Sulfonated cyanines Lumiprobe explains

Sulfonated and non-sulfonated cyanines exhibit very similar fluorescent properties. Non-sulfonated cyanines must be dissolved in organic co-solvent (DMF or DMSO) prior to use, and added to a solution of target molecule in aqueous buffers. Recommended volume of co-solvent should be 10% for Cy3, Cy5, Cy7, and 15% for .5 counterparts. Sulfo-Cy reagents can be used in purely aqueous conditions. There is also a difference in purification: when dialysis against water or aqueous buffer is used for purification, sulfo-Cy must be used to achieve efficient removal of unreacted dye material. Reactions with both sulfo- and non-sulfo cyanines can be purified by gel filtration, chromatography (HPLC, FPLC, ion exchange), or electrophoresis.

Sulfonated and non-sulfonated cyanines are interchangeable for the labeling of many classes of targets including:
* soluble proteins, which are tolerant to addition of organic co-solvent
* antibodies (use 5-10% of DMSO/DMF)
* DNA and oligonucleotides
* peptides
* many small molecules

continue reading what and how to use http://www.lumiprobe.com/tech/cyanine-dyes .... as thank you for reviewing - a 5% discount , use discount code: microbeworld
Lumiprobe.com offers cyanine dyes and explains the difference. Free Tech support is available to help you determine what to use for your research.
 
 

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