This is simlpe phenotypic test which can be done in simple laboratory where molecular technology is not easily avaialble.phenotypic detection of MBL by 0.1mM EDTA.metallo-β-lactamase(MBL )is ambler's class B beta lactamase,which is inhibited by EDTA.this research work was done in our research lab., department of clinical microbiology,tribhuvan university teaching hospital in nepal.
RESULT: zone of inhibition of IMIPENEM with EDTA(IMP+EDTA) is greater than IMIPENEM (IMP) alone by 5mm.
MEDIA : mulluer hinton agar .
ORGANISM: PSEUOMONAS AEUGINOSA.so this organism is MBL producer.
by CLS SUNDAR KHADKA,
post graduate in clinical microbiology,institute of medicine(IOM),TEACHING HOSPITAL,KTM,NEPAL.