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phenotypic detection of MBL by EDTA.

cls sundar.jpg
phenotypic detection of MBL by EDTA.MBL is ambler's class B beta lactamase,which is inhibited by EDTA.this research work was done in our research lab., department of clinical microbiology,tribhuvan university teaching hospital in nepal.
RESULT: zone of inhibition of IMIPENEM with EDTA(IMP+EDTA) is greater than IMIPENEM (IMP) alone by 5mm.
MEDIA : mulluer hinton agar .
ORGANISM: PSEUOMONAS AEUGINOSA.so this organism is MBL producer.

by CLS SUNDAR KHADKA,
post graduate in clinical microbiology,institute of medicine(IOM),TEACHING HOSPITAL,KTM,NEPAL.
 
 

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