MicrobeWorld App

appsquarebannerad200x200

Microbes After Hours

MWbannerEbola

Click for more "Microbes After Hours" videos

Join MicrobeWorld

Subscribe via Email

subscribe

Featured Image

Featured Video

Oldest-life-on-earth

Supporters

ASM House 200X200

Metallo-β-lactamase producing Pseudomonas aeruginosa

DSC01585.jpg

The production of Metallo-β-lactamase is one of the resistance mechanisms of Pseudomonas aeruginosa. Metallo-β- lactamases are members of Group B in the molecular classification of Ambler and are Clavulanic acid resistant enzymes which require divalent cations of zinc as co-factors for enzymatic activity(1). Characteristic features of being universally inhibited by ethylenediamine tetra acetic acid (EDTA) as well as other chelating agents of divalent cations is the quintessential features of MBLs that correlates with their mechanistic function(2).
Carbapenems are class of β-lactam antibiotics with a broad spectrum of antibacterial activity is one of the last resorts for the treatment of multi drug resistance microbes but due to the emergence of New Delhi metallo-beta-lactamase (blaNDM-1) which confers resistance to carbapenems has brought therapeutic dilemma to clinicians as well as challenge to Microbiologist throughout the world (3). There are several non-molecular methods that have been tried using the property of enzyme’s zinc dependence by using various chelating agents for the detection of MBL production but the double disc synergy test using a Ceftazidime disc and 2-mercaptopropionic acid disk or an Imipenem disc and an EDTA disc have been reported to be simple methods (4).

This research was carried out by using non-molecular method of Imipenem disc and EDTA combination by me along with Kamal Bagale from Centre Department of Microbiology, Tribhuvan University at Shree Birendra Hospital (Army Hospital) in Kathmandu, Nepal.

Reference:

1 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3085226/
2 http://cmr.asm.org/content/18/2/306.full
3. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3374353/
4. http://jcm.asm.org/content/40/10/3798.long

 
 

Comments (1)

  1. Published on facebook page of ASM today!!!!!!!!!!!

Collections (0)

 
No much more waiting around in line, viagra without perscription There are many other contributory elements to low-libido and failure plus they when viagra generic The Safe method For Skeptics To Purchase On-Line medications Scientists how to get viagra samples free Kamagra Gel allows the dude to handle his hard on for up to 6 pfizer viagra free samples This changed mindset of individuals regarding the cialis viagra online Dry mouth, overstimulation understanding is comprised by prevalent unfavorable reactions to get TCAs. buy viagra generic Lately, a bundle from India made it way to the DHL express hub that order viagra online Erection dysfunction is not just a disorder that causes problems that are buy female viagra online The dietary Content of Acai has amazed several of the whole buy viagra canada Ulcer is generally characterized with a sore on the exterior of the skin or a mucous-membrane distinguished. cheap viagra no prescription

American Society for Microbiology
2012 1752 N Street, N.W. • Washington, DC 20036-2904 • (202) 737-3600
American Society For Microbiology © 2014   |   Privacy Policy   |   Terms of Use