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Multidrug resistance Pseudomonas aeruginosa

Pseudomonas aeruginosa is one of the most notorious organisms that have brought the therapeutic dilemmas to clinicians as well as challenge to microbiologist throughout the world. It carries gene of intrinsic resistance (AmpC cephalosporinase, the OprD outer membrane porin, and the multidrug efflux pumps) furthermore imported resistance to the lactams involves the production of inactivating lactamases enzyme and to aminoglycosides involves production of aminoglycoside-modifying enzymes and methylation of the 16S rRNA. Fluoroquinolone resistance among P. aeruginosa isolates has been linked only to chromosomal genes, with mutational changes in the fluoroquinolone targets DNA gyrase (gyrA and gyrB) and/or topoisomerase IV (parC and parE) and/or overexpression of multidrug efflux pumps.

Further information: http://cmr.asm.org/content/22/4/582.full

The isolated Pseudomonas aeruginosa was found resistance to Ceftazidime (30 mcg) Piperacillin(100mcg) Amikacin(30mcg) Aztreonam(30mcg) Cefepime(30mcg) Ciprofloaxacin(1mcg) Gentamicin(10mcg) Piperacillin /Tazobactem(100/10mcg) Imipenem (10mcg) Ofloxacin(5mcg) while sensitive to Colistin (25mcg) and Polymyxin B(300units) .

Photograph was taken in the Microbiology lab of Shree Birendra Hospital (Army Hospital) at Kathmandu, Nepal.

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