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Tuberculosis genomes recovered from 200-year old Hungarian mummy

Researchers at the University of Warwick have recovered tuberculosis (TB) genomes from the lung tissue of a 215-year old mummy using a technique known as metagenomics.

The team, led by Professor Mark Pallen, Professor of Microbial Genomics at Warwick Medical School, working with Helen Donoghue at University College London and collaborators in Birmingham and Budapest, sought to use the technique to identify TB DNA in a historical specimen.

The term ‘metagenomics’ is used to describe the open-ended sequencing of DNA from samples without the need for culture or target-specific amplification or enrichment. This approach avoids the complex and unreliable workflows associated with culture of bacteria or amplification of DNA and draws on the remarkable throughput and ease of use of modern sequencing approaches.

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