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Tasha - Comments

A cute smile. It is good to DIY something interesting during one's work. Candy Swift Biolabs Blog http://www.creative-biolabs.com/blog
We isolated a similar organism from a backpack a couple years ago but we were unsuccessful at identification. I am interested to see if anyone has any ideas?
I'd like to recommend this site: http://www.creativebiomart.net/bacterial-expression-systems.htm which is related to e.coli.
Interesting. It makes me smile too.
I'm thinking about replicating this with one or two pre-sterilized Mueller-Hinton agar and use MALDI to try and identify some of them.
Good to have fun sometimes.
This made me smile.
Thanks! In a pinch when our autoclave is down I have boiled the media for about 15-20 min kind of like home canning....just be careful because it will boil over! If you have a bottle that will withstand the boiling then use that and sterilize the lid with bleach and let that dry. Once you have boiled the media put the lid on, loosely, and let it cool until you can hold it in you hand comfortably, then pour. Try to pour the plates in a room without and lot of airflow so you don't get to many contaminants. I must caution that once you grow anything on the plates to consider that plate a Biohazard and make sure it is disposed of properly. Contact your local College, Dr, Vet, Dentist, etc and see if they can dispose of it for you. And never open the plate once things have grown. Tape the lid shut so there are no accidents. You can also put it in a ziplock and zipper it shut.
Love this! For us trying to do this at home, is it necessary to use an autoclave to sterilize the TSA (240 deg F with pressurized steam)? Would a home oven at 240 deg F without the steam be sufficient? Thanks for posting this. By the response you've had you can tell that lots of us think this is the coolest thing!
Tasha, My name is Kristine Vester and I am an educator at the MathScience Innovation Center in Richmond, VA. We write different STEM lessons and presentations for the Central Richmond Area grades K-12. I would love to use your image in a couple of our activities, posting it online for teacher use. Could I get your permission to post your picture online for our teachers to use? Thanks so much.
This is super cool. Thanks for the step-by-step. I am going to find someone with lab access so i can play this game with my kids... (12 &14 this summer).
This inner-shape pattern is formed for material transport . . . it seems to be a distribution system
quoted....not quotes.....
I'm working on a food safety video for students in the Kansas State University Department of Hospitality Management and Dietetics. I would like permission to use your photo as a dramatic way to impress students going into the food service industry, the necessity of hand washing in all phases of food handling and meal preparation. This is a non-commercial educational video. I'm happy to answer additional questions! Ron Frank, Emeritus Professor, Kansas State University Department of Communications rfrank@ksu.edu
Sarah, I just answered a min ago.....I have been getting blitzed by the media and have spent several hrs this morning answering questions or responding to emails. Believe it or not that is not a scope image rather taken with a macro lens.
I didn't realize Tasha had answered this question previously, oops! If the OP has seen it under a scope, I'd take their word for it first :)
The large blob on the lower right is more than likely a Bacillus, see cloes up image: http://www.microbeworld.org/component/jlibrary/?view=article&id=13869 Looks like it out grew everything around it and that is also an area on the plate( the palm) that may not have gotten the contact because of the conture of the hand.
bharat, the large colony on the lower right part of the plate looks like Streptomyces. They're commonly found in soil, and form large, irregular colonies with fungal-like mycelial growth patterns. They're also well-known for producing an array of antibiotics (hence the lack of other growth near that colony). I used to take LB plates home for my daughter and she'd find all sorts of things (leaves, rocks, twigs) and make prints with them. TSA gives a much more colorful display! Very cool!
Hi! My name is Christine and I am with Newsy.com. We are thinking of working on a story about your son's handprint! Could we have permission to use this photo in our story? slusser@newsy.com. Thank you!
Which is the large colony on the right side bottom of the plate. I see there are no colonies close to this colony. Any guess?
http://www.theblaze.com/stories/2015/06/08/8-year-olds-bacteria-handprint-shows-whats-lurking-out-in-the-world-most-of-it-wont-hurt-you/
And this is the first of the interviews on NBC related to this image: http://www.today.com/health/striking-photo-shows-all-bacteria-8-year-olds-hand-t25096 Thank you ASM for the opportunity to post images like this!
I remember years ago having a student with a child in kindergarten. Any time he was near campus, he would beg his father to take him to the microbiology laboratory, where he would beg me for a plate. He'd then touch the plate and take it home. Later, his father would bring it back to be autoclaved. Anyway, the kid just thought it was the coolest thing. We microbiologists should appreciate the appeal that our discipline can have to young children. A director of a Montessori school that used to bring their children to my lab speculated that it may be microbes' relative invisibility and possible danger that makes them irresistible. Find a way to get little kids into the lab. You'll make their day, and they'll make yours. (By the way, found this post as a link from Vox!)
I am saving the plate to show the micro students in 1 1/2 weeks. I did take a few close up photo's of some colonies which were also posted: http://www.microbeworld.org/component/jlibrary/?view=article&id=13870 and http://www.microbeworld.org/component/jlibrary/?view=article&id=13869 The big blob in the lower right is the image "unknown from handprint #2" and is most likely a Bacillus sp which is commonly found in dirt.....saw a fair # of what looks like Bacillus. The other image is either a yeast or Bacillus as well. We do this at the beginning of the semester and this is pretty common. The white being Staph and the colored colonies probably yeast. The colored colonies tend to show up after the plates are refrigerated or left at room temp for a few days. Once summer school starts I might do some G stains if I have time but micro is not the only class I tech and it looks to be a fairly busy summer.
This is the coolest thing I have ever seen. Did you test this to see which bacteria are present? I am interested to know if the ones shown are good flora naturally present on the hands.
Look at this site: https://catalog.hardydiagnostics.com/cp_prod/Hardy_Part_Description.aspx these are the sterile plates I used. Tryptic Soy Agar (TSA) can be purchased through a number of companies (Fisher Scientific, Hardy Diagnostics, Neogen). Most sell it in the powdered form, add water, autoclave, cool to about 55 degrees then pour into the plate, cover with the lid then let solidify. Once the plates are cool then place the hand on the plate making sure to gently pressing the fingers/palm to make contact with the agar. Cover the plate with the lid and place in a 37 degree C incubator for 24-48 hrs......incubate agar side up. This will grow the normal flora on the hand like Staph., Micrococcus, etc. Take the plate out and let it incubate/set out with the lid on at room temp (22 degrees C) for several days (3+ days). Normal flora will continue to grow (slowly) and yeast/fungi will start to grow....usually colored colonies (red/pink/yellow). It will also help bacteria like Serratia turn red. Once grown the plate should be treated as a Biohaz and disposed of properly. The plate should not be opened if mold/fungi is present without proper respiratory protection. Hope this helps.
OK, I am not familiar with this process, so let me clarify: You used a regular, sterilized (?) dinner plate, and poured the TSA material onto it, making a big petri dish? Where can I get the TSA liquid (?) to make one, and how do I culture the bacteria? Warm oven? Covered/uncovered? A step by step would be appreciated! It makes for a wonderful piece of photographic/scientific art, and a great learning tool!!!
Thanks! I used a large Kirby Bauer plates (15 x 150 mm) with TSA. I pour the plates myself and get them from either Hardy or Fisher.
How can I construct or purchase a plate to make a handprint of my child? Your image is gorgeous!!!
Yeah but its fun trying!
Ubiquitous! Doubt if we can ever meet them all... Seems they evolve in milliseconds.
Ubiquitous! Doubt if we can ever meet them all... Seems they evolve in milliseconds.
Yes, I just took a look and saw the comments. It does look different that what I saw in March. We have a lot of mushrooms out here on the west cost especially in the Redwoods. Some edible and some deadly so I would NEVER eat one unless I had an expert along. We do have a co called Monterey mushrooms, http://www.montereymushrooms.com/about-us/about-monterey-mushrooms/, and I bet if you sent them the photo they would be able to give you definate answer. Either way would not eat it.......
Tasha aka Sturm, have you seen the Facebook conversation about this? They are saying Laetiporus sulphureus or cincinnatus, aka "chicken of the woods"
Very cool! Thanks for posting.
A fungal cotaminant on MRS Agar after 15 days at refrigerated temperatures, means I lost my sample 'lactic acid bacteria' Ps: the picture is not clear
I would assume Bacillus as well. I am hoping to run a gram stain on some time next week.
Seems like a swarming Bacillus
This media is usually used to differentiate between Strep and Enterococcus but I believe Staphlococcus will grow as well since they are salt tolerant as well.
What other microbes can to grow in this medium?
just posted as ASM's pic of the day. thank you!
MacConkey......
Correction: S. aureus is fine turbidity not flocculent. Flocculent would look more like flakes throughout the tube.....almost like a snow globe. Apologies.
Seems to be a glitch, the image did not come through on this post try: http://www.microbeworld.org/component/jlibrary/?view=article&id=12039
Thanks for the great pic!! It was just posted as ASM's pic of the day on our Facebook and Instagram account.
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