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Click-Chemistry Labeling of Oligonucleotides and DNA - Lumiprobe - Protocol


Click chemistry is a versatile reaction that can be used for the synthesis of a variety of conjugates. Virtually any biomolecules can be involved, and labeling with small molecules, such as fluorescent dyes, biotin, and other groups can be readily achieved.

Click chemistry reaction takes pl... Read More

Protecting against Hendra virus

It was discovered in 1994 as a virus 'hosted' by fruit bats and lethal to horses and humans. Watch our profile of the Hendra virus, a zoonotic disease that has claimed the lives of seven people. Read More

Sizing up bacteria

A new theoretical framework outlined by a Harvard scientist could help solve the mystery of how bacterial cells coordinate processes that are critical to cellular division, such as DNA replication, and how bacteria know when to divide.

For decades, scientists have believed that cellular divis... Read More

Intestinal Bacteria Influence Food Transit Through the Gut

Food transit through the small intestine affects the body's absorption of nutrients and, consequently, our health. The discovery that food transit time is regulated by a hormone indicates new ways to increase the intestinal absorption of nutrients, and thus potentially treat malnutrition.

One... Read More

FrameBot: a new tool for those pesky sequencing problems

In their paper in mBio this week, Wang et al present FrameBot, a program that combines frameshift correction and nearest-neighbor identification in one Java-based tool. Read More

Where ‘Nightmare Bacteria’ Came From, And How Our Inattention Helped Them Emerge

Cast your minds back a few months ago, to when the director of the US Centers for Disease Control and Prevention announced, “We have a very serious problem” with “nightmare bacteria,” and the chief medical officer of the United Kingdom backed him up a few days later, describing a “ticking time b... Read More

When bacteria fight back

Q&A with Dr. David Hooper on the rising threat from drug-resistant microbes. Read More

Choosing cheese: Research identifies microbial communities in cheese

After studying 137 varieties of cheese collected in 10 different countries, researchers have been able to identify three general types of microbial communities that live on cheese, opening the door to using each as a 'model' community for the study of whether and how various microbes and fungi c... Read More

From Geology to Biology: A Serpentine Story of Early Life

Over 4.5 billion years ago, the Earth was a superheated sphere of molten rock, radiating heat to space at over 2000 K. A billion years later, it had global oceans, teeming with microorganisms. In that time, the Earth underwent massive geological changes, somehow serendipitously creating conditio... Read More

Antibiotics improve growth in children

Antibiotics improve growth in children at risk of undernourishment in low and middle income countries, according to researchers at McGill University who have just conducted a research literature review on the subject. Their results, published in the British Medical Journal, suggest that the youn... Read More

Botulism Bacteria Blamed for Deaths of Waterbirds on Lake Ontario

Last year, I wrote about finding the carcasses of dead loons and cormorants on the shores of a Northern Lake Michigan beach. Now officials in New York are reporting similar waterbird deaths from Type E botulism on Lake Ontario.

Sadly, finding type E botulism in waterbirds is becoming an “annu... Read More

Structure of bacterial nanowire protein hints at secrets of conduction (w/ Video)

Tiny electrical wires protrude from some bacteria and contribute to rock and dirt formation. Researchers studying the protein that makes up one such wire have determined the protein's structure. The finding is important to such diverse fields as producing energy, recycling Earth's carbon and min... Read More

The U.S. Neglects Its Best Science Students

The U.S. education policy world—the entire country, for that matter—is on a quest to increase the ranks of future innovators in science and technology. Yet the programs that get funded in K–12 education do not support students who are already good at and in love with science. These students have... Read More

Poop pill may treat stubborn, deadly C. diff bacteria

Transplanting fecal matter has been one of the best remedies at treating a tough bacterial infection known as Clostridium difficile (C. diff). Scientists are now saying they can give all the benefits of poop in a tiny pill.

This new pill method is a less yucky way to do "fecal transplants." A... Read More

Plasmas attack bacterial cells on several levels

As they destroy bacteria very efficiently, plasmas constitute an alternative to chemical disinfectants and potentially to antibiotics, as well. How they achieve this effect has been investigated by biologists, plasma physicists and chemists at the Ruhr-Universität (RUB). Cold atmospheric-pressur... Read More

IDing Livestock Gut Microbes Contributing to Greenhouse Gas Emissions

“Increased to levels unprecedented” is how the Intergovernmental Panel on Climate Change (IPCC) described the rise of carbon dioxide, methane and nitrous oxide emissions in their report on the physical science basis of climate change in 2013. According to the US Environmental Protection Agency ... Read More

Scientists Add Letters to DNA’s Alphabet, Raising Hope and Fear

Scientists reported Wednesday that they had taken a significant step toward altering the fundamental alphabet of life — creating an organism with an expanded artificial genetic code in its DNA.

The accomplishment might eventually lead to organisms that can make medicines or industrial product... Read More

Life on cheese: Scientists explore the cheese rind microbiome

Bacteria and moulds are vital to the ripening and aroma of many cheeses. Scientists from the Institute for Milk Hygiene, Milk Technology and Food Science at the University of Veterinary Medicine, Vienna are working to identify the microorganisms that live on the rind of Vorarlberger Bergkäse, an... Read More

ASM GM 2014 - Windshield Wiper Fluid: A Source of Legionnaires?

A form of bacteria responsible for respiratory illness, including the deadly pneumonia known as Legionnaire's disease, may be able to grow in windshield washer fluid and was isolated from nearly 75% of school buses tested in one district in Arizona. The participant will discuss findings from ... Read More

Learning from a virus: Keeping genes under wraps

By studying how a virus that infects most people at some point in their lives packages its genetic material during infection, an international collaboration of researchers has made discoveries that help scientists better understand virus-host interactions and may open new avenues for therapies.
... Read More

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