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Klebsiella pneumoniae

Large (about 5 mm in diameter), lactose positive colonies of Klebsiella pneumoniae on desoxycholate-citrate agar. Cultivation 37°C, 24 hours.

Klebsiella pneumoniae is a common source of hospital-acquired infections. Some of the strains can carry plasmids that harbour genes conferring resistan... Read More

E. coli gram stain

Gram stain of E. coli showing characteristic G- rods. Read More

Euglena mutabilis

Living green alga Euglena mutabilis. Technique: Differential interference contrast. Credit: Gerd Gunther, Düsseldorf, Germany

Nikon Small World 2012 Honorable Mention. Read More

TWiM #78: A bacterium grows in Brooklyn

Hosts: Vincent RacanielloElio Schaechter, and Michael Schmidt.


Vincent, ... Read More

BacterioFiles Micro Edition 114 - Popping Pathogen Provides Plasmid Prophylactic

This episode: Salmonella strain engineered to induce our cells to immunize us against diseases!


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Download Episode&... Read More

Bespoke microbiome therapy to manage plant diseases

In this paper we describe the perspective of following the achievements in medicine, where gut microbiome transplants are proving to be a powerful method to overcome several gastrointestinal disorders and many other physiological problems, to possible solve diseases in plants. There is strong si... Read More

TWiP 63: Plasmodium of the apes

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Hosts: Vincent Racaniello and Dickson Despommier


Vincent and Di... Read More

Using Wordcloud-Reponses to Discover How Students Perceive Concepts in the Classroom

In this blog, I share some "first word that comes to mind" responses of my freshman biology class to the words "germ," "bacteria," "cell," and "DNA." The way that we perceive an idea or concept definitely impacts our relationship with it. Thus, finding out what students think, coming into our ... Read More

Fungus on toilet seat

Culturing microbes on nutrient plate and testing for different kind of microbes on specific environment areas. This inoculated nutrient plate that was left for a period of 14 days showed the present of a few different kind of fungus species. It’s common to think that the human palm contains more... Read More

BacterioFiles Special Edition - ASM2013 General Meeting Day 1

I went to the General Meeting of the American Society for Microbiology, in Denver, Colorado, and I wanted to share some of the fascinating science that I experienced. So here's my summary of the first day!


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BacterioFiles Micro Edition 136 - Swarmers Send Secret Signals

This episode: Swarming predatory bacteria may be communicating through tubes!


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Download Episode (6.3 MB, 6.9 minutes... Read More

TWiM #93: Worming in on bacteria

Hosts: Vincent RacanielloMichael Schmidt, ... Read More

TWiM #99: Careers in Biodefense

Hosts: Vincent Racaniello


Guests: Maria Julia Marinissen, Read More

TWiP 71: Happy trails to you

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Hosts: Vincent Racaniello and Dickson Despommier Read More

BacterioFiles 159 - Drastic Diet Develops Different Dwellers

This episode: Gut microbe communities can change rapidly to accommodate major diet changes!


(12.2 MB, 13.3 minutes)


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Scientific American editor Fred Guterl discusses viruses and the H5N1 controversy on Jon Stewart's Daily Show

In this clip from Comedy Central's The Daily Show with Jon Stewart, guest Fred Guterl, award-winning journalist and executive editor of Scientific American, discusses his new book, "The Fate of the Species,"and touches on viruses, influenza, scientific research, and the recent H5... Read More

Endospore Stain Bacillus cereus

B. cereus was grown on urea agar for 6 days at 37 degrees C to induce spore formation.
The endospore stain was done using malachite green as the primary stain and safranin as counter the stain. Malachite green was applied to the smear and set over a steaming water bath for 10 min (stain was no... Read More

TWiV 280: Post viral

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Hosts: Vincent Racaniello, Dickson Despommier Read More

TWiV 273: Lambda is not just a phage

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Hosts: Vincent Racaniello, Dickson Despommier, Read More

Cyanine dyes - Non-sulfonated and Sulfonated cyanines Lumiprobe explains

Sulfonated and non-sulfonated cyanines exhibit very similar fluorescent properties. Non-sulfonated cyanines must be dissolved in organic co-solvent (DMF or DMSO) prior to use, and added to a solution of target molecule in aqueous buffers. Recommended volume of co-solvent should be 10% for Cy3, C... Read More
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